Going high-throughput: Dynamic conformations and interactions in vivo.
Proteins are intrinsically dynamic molecules that continuously explore conformational ensembles to execute biological functions. Conventional structural biology methods rely on in vitro reconstitution of purified components and therefore capture predominantly static snapshots, often overlooking the…
Systematic identification of bacterial neuraminidase inhibitors from Psoralea corylifolia using ultrafiltration-UPLC-Q-Orbitrap-MS and molecular dynamics.
Bacterial neuraminidase (BNA) is a potential target for discovering anti-infective agents. Here, the BNA inhibitory activity of the ethanolic extract of Psoralea corylifolia (EEPC) was investigated using integrated experimental and computational approaches. Affinity ultrafiltration-UPLC-Q-Orbitrap-M…
Deciphering soybean-microbiome interactions: from rhizosphere dynamics to sustainable yield enhancement.
The soybean plant (Glycine max L.) is an important crop for valuable food source because of its high levels of protein and oil, thus contributing greatly to a sustainable system for producing food through biological nitrogen fixation. Recent research supports the theory that the soybean-associated m…
Posttraumatic growth and depreciation among Ukrainians: a structural equation modelling approach.
Background: War-related stressors can disrupt core beliefs and shape trauma outcomes, yet the distinct roles of moral processes in post-traumatic outcomes remain insufficiently understood.Objective: To examine how war-related stressors (WRS) and core-belief disruption (CBD) relate to posttraumatic s…
Identification and profiling of HLA-A*02:01-restricted Toxoplasma gondii peptides through immunopeptidomics in HLA-A2.1 transgenic mice.
HLA class I presentation of pathogen-derived peptides is essential for CD8+ T-cell recognition of Toxoplasma gondii. While in vitro MHC ligands have been documented, the in vivo ligandome during infection progression remains poorly characterized. Here, we employed an MS-based immunopeptidomics appro…
Aggrecan and polymeric immunoglobulin receptor in extracellular vesicles of patients with seropositive rheumatoid arthritis.
The presence or absence of autoantibodies distinguishes patients with rheumatoid arthritis (RA) as seropositive (SP) or seronegative (SN), with SP generally associated with poorer prognosis. Extracellular vesicles (EVs) are important mediators of intercellular communication and may contribute to RA…
AI-assisted MALDI-TOF MS for identifying carbapenem resistance in clinical Acinetobacter baumannii isolates.
Carbapenem-resistant Acinetobacter baumannii (CRAB) is one of the most critical public health threats worldwide due to its high infection rates, substantial mortality, and limited therapeutic choices. As CRAB infections are frequently multidrug-resistant, rapid and accurate determination of carbapen…
Immune cell-derived membrane nanovesicles: A promethean fire for autoimmune disease therapy through immune cell mimicry.
Autoimmune diseases (AIDs) constitute a heterogeneous group of disorders characterized by immune dysregulation, loss of self-tolerance, and chronic inflammation, which leads to tissue damage and organ dysfunction. Current therapies for AIDs are often limited by their lack of specificity, systemic si…