Going high-throughput: Dynamic conformations and interactions in vivo.
Proteins are intrinsically dynamic molecules that continuously explore conformational ensembles to execute biological functions. Conventional structural biology methods rely on in vitro reconstitution of purified components and therefore capture predominantly static snapshots, often overlooking the…
Pre-exposure to UV-B irradiation induces antioxidant enzymes and mitigates Fusarium acutatum stress in shallot (Allium cepa L.).
Shallot (Allium cepa var. aggregatum) productivity is often limited by fungal diseases, particularly twisted disease caused by Fusarium acutatum. This study evaluated the effects of UV-B pre-exposure (150 min) on enhancing plant resistance under greenhouse conditions using four treatments: co…
Complement C3 inhibitory peptide AMY-101 ameliorates brain injury in a mouse model of NMOSD.
Neuromyelitis optica spectrum disorder (NMOSD) is an autoimmune inflammatory demyelinating disease of the central nervous system, clinically characterized primarily by optic neuritis and transverse myelitis. This study aimed to investigate the therapeutic potential of the complement C3 inhibitory pe…
Sex- and age-specific associations of VEGFA polymorphisms with multiple sclerosis susceptibility.
To evaluate six VEGFA polymorphisms (rs1570360, rs699947, rs3025033, rs2146323, rs1413711 and rs833061) and serum VEGFA concentrations in 270 Lithuanian patients with multiple sclerosis (MS) and 270 matched healthy controls. Genotyping was performed using real-time PCR, and serum VEGFA levels were m…
Systematic identification of bacterial neuraminidase inhibitors from Psoralea corylifolia using ultrafiltration-UPLC-Q-Orbitrap-MS and molecular dynamics.
Bacterial neuraminidase (BNA) is a potential target for discovering anti-infective agents. Here, the BNA inhibitory activity of the ethanolic extract of Psoralea corylifolia (EEPC) was investigated using integrated experimental and computational approaches. Affinity ultrafiltration-UPLC-Q-Orbitrap-M…
Deciphering soybean-microbiome interactions: from rhizosphere dynamics to sustainable yield enhancement.
The soybean plant (Glycine max L.) is an important crop for valuable food source because of its high levels of protein and oil, thus contributing greatly to a sustainable system for producing food through biological nitrogen fixation. Recent research supports the theory that the soybean-associated m…
Posttraumatic growth and depreciation among Ukrainians: a structural equation modelling approach.
Background: War-related stressors can disrupt core beliefs and shape trauma outcomes, yet the distinct roles of moral processes in post-traumatic outcomes remain insufficiently understood.Objective: To examine how war-related stressors (WRS) and core-belief disruption (CBD) relate to posttraumatic s…
Identification and profiling of HLA-A*02:01-restricted Toxoplasma gondii peptides through immunopeptidomics in HLA-A2.1 transgenic mice.
HLA class I presentation of pathogen-derived peptides is essential for CD8+ T-cell recognition of Toxoplasma gondii. While in vitro MHC ligands have been documented, the in vivo ligandome during infection progression remains poorly characterized. Here, we employed an MS-based immunopeptidomics appro…